ENVIRONMENTAL CONTROL IN CRISPR AND GENE EDITING LABORATORIES: A Comprehensive Technical and Regulatory Guide for Laboratory Design, Biosafety, and Operational Excellence

The environmental control principles described in this document apply broadly to Biosafety Level 1 (BSL-1), BSL-2, and BSL-3 laboratories engaged in genetic research, with specific emphasis on facilities performing CRISPR-Cas9, base editing, prime editing, and related genome engineering activities.

This guide does not replace site-specific risk assessments, institutional biosafety committee (IBC) review, or regulatory submissions. All facility designs and operational protocols must be reviewed and approved by the relevant competent authorities before implementation.