The ultimate goal of this book is to assist learners, including students, food analysts, and pharmaceutical technicians, in understanding method development, method validation, and troubleshooting with practical examples and proper experimental layouts. The content and treatment of the subject matter are considered most appropriate for university students studying chromatography who wish to learn how to use data for various parameters such as linearity, repeatability, limit of quantitation (LOQ), and limit of detection (LOD). True data are illustrated in Excel spreadsheets, and solutions are provided in a stepwise manner to ensure that all stakeholders can properly grasp the concepts. Concepts such as theoretical plates, plate height, resolution, selectivity, and the Van Deemter equation are demonstrated with practical data, and solutions are addressed in a simplistic manner. The ICH (International Consortium for Harmonization), SANTE guidelines, and other regulatory compliances have been intentionally incorporated to facilitate the NABL accreditation of laboratories across the country. Measures of Uncertainty (MoU) are given full attention and exemplified in Excel datasheets so that all PG students and other personnel can become acquainted with the methodology used in elucidating the MoU.
Prof. Asim Biswas is an accomplished academic, currently holding the prestigious OAC Chair in Soils and Precision Agriculture at the School of Environmental Sciences, University of Guelph, Canada. He serves as a Professor and the Deputy Director at the same institution and has visiting professorships at three other universities. With various leadership roles in professional societies, Prof. Biswas is actively involved in advancing his field. His research focuses on sustainable soil management, aiming to improve productivity and resilience in land-based agri-food systems while considering environmental sustainability and climate change. Through innovative integration of soil data, sensors, and technology, Prof. Biswas provides strategies for environmentally and financially sustainable agricultural productivity. Leading a well-funded research team, he has published extensively (e.g., >220 peer reviewed journal paper including in Nature Reviews, 22 book chapters, 8 edited books, >225 conference papers, and a granted patent), delivered keynote speeches, and serves as an editor for multiple (8) journals. Additionally, he teaches multiple undergraduate, graduate, and special courses.